FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34935465
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3744291.0717733397852296TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1061800.045.173319
AGAGCAC1061450.045.1214038
TCGTATG497050.044.1567544-45
CGTATGC509650.043.7822146-47
TATGCCG514100.043.36097348-49
TCGGAAG1110400.043.124313
ATCGGAA1110400.042.957782
CTCGTAT473400.042.7454244-45
CGGAAGA1129350.042.3718034
GATCGGA1137900.041.1336481
TCTCGTA488350.040.85354242-43
AATCTCG495350.040.1426340-41
AAGAGCA1203800.039.829237
ATCTCGT503550.039.47413342-43
ATGCCGT572900.038.7696448-49
GTATGCC586350.038.0388946-47
GCCGTCT581450.037.01020450-51
GAAGAGC1326450.036.265646
CCGTCTT598650.036.00218652-53
TGCCGTC621250.035.6751750-51
Produced by FastQC (version 0.11.5)