FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33681741
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3412691.0132166267771014TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG451600.044.15153544-45
CGTATGC464000.043.51359646-47
TATGCCG464350.043.47503748-49
AGAGCAC1000600.043.193538
GAGCACA1008700.042.922099
CTCGTAT435150.042.682244-45
TCTCGTA448950.040.94216542-43
TCGGAAG1063050.040.64373
ATCGGAA1066050.040.3382072
CGGAAGA1074050.040.241134
AATCTCG461050.039.6573940-41
ATCTCGT466000.039.2046242-43
GATCGGA1092800.038.5757751
ATGCCGT521050.038.49799748-49
GTATGCC537250.037.73557346-47
AAGAGCA1150250.037.5740137
GCCGTCT528850.036.9100250-51
CCGTCTT546300.035.83523652-53
TGCCGTC568000.035.093550-51
GAAGAGC1260650.034.443196
Produced by FastQC (version 0.11.5)