FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37918719
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3680510.9706314182185322TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG488350.043.80024344-45
CGTATGC499250.043.54723446-47
AGAGCAC1076950.043.095468
GAGCACA1081850.043.0493749
TATGCCG506250.042.9446548-49
CTCGTAT466300.042.4639344-45
ATCGGAA1122050.041.2719842
TCGGAAG1127550.041.251113
CGGAAGA1148900.040.5094534
TCTCGTA485100.040.31453342-43
AATCTCG489400.039.75237340-41
GATCGGA1136400.039.6843031
ATCTCGT505800.038.60830342-43
ATGCCGT567100.037.909548-49
AAGAGCA1249250.037.3266647
GTATGCC585100.037.1414446-47
GCCGTCT583800.035.90514850-51
CCGTCTT603700.035.08337852-53
TGCCGTC624550.034.4788950-51
GAAGAGC1370400.034.156226
Produced by FastQC (version 0.11.5)