FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32858684
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3889471.1836962186312756TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1012700.049.0520259
AGAGCAC1016550.048.805588
ATCGGAA1035550.047.8016932
TCGGAAG1043650.047.6983573
CGGAAGA1067800.046.5352754
GATCGGA1052300.046.3148881
TCGTATG517750.044.53293644-45
CGTATGC530850.044.1315946-47
TATGCCG534750.043.809448-49
CTCGTAT493950.043.3080744-45
AAGAGCA1154450.043.1816337
TCTCGTA506950.041.67338642-43
AATCTCG509350.041.0722540-41
ATCTCGT523750.040.11898442-43
GAAGAGC1254650.039.8058626
ATGCCGT585600.039.6199748-49
GTATGCC604650.038.74122246-47
GCCGTCT598050.037.6272250-51
CCGTCTT608450.037.27263652-53
TGCCGTC634350.036.5036350-51
Produced by FastQC (version 0.11.5)