FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35118480
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4000441.1391267503604938TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1060950.047.1240582
AGAGCAC1069500.046.7447288
TCGGAAG1074050.046.7030223
GAGCACA1076650.046.4958659
GATCGGA1072050.045.921861
CGGAAGA1100800.045.4262964
TCGTATG525700.044.1574944-45
CGTATGC538050.043.81450746-47
TATGCCG541850.043.59897648-49
CTCGTAT499700.042.97116544-45
AAGAGCA1213350.041.285487
TCTCGTA515850.041.25337642-43
AATCTCG521800.040.60206240-41
ATGCCGT595850.039.52815648-49
ATCTCGT536850.039.4582842-43
GTATGCC615100.038.44195646-47
GAAGAGC1317400.038.1703346
GCCGTCT606900.037.810150-51
CCGTCTT621450.037.03934552-53
TGCCGTC649050.036.12309350-51
Produced by FastQC (version 0.11.5)