FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29693129
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3493371.1764910326560734TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG474400.044.37685444-45
CGTATGC488000.043.7962346-47
TATGCCG494550.043.3169548-49
CTCGTAT452800.042.76441244-45
TCTCGTA463600.041.25112542-43
GAGCACA1124050.040.6808139
AATCTCG467400.040.6114840-41
AGAGCAC1129950.040.3424958
ATCTCGT478000.039.79475842-43
ATGCCGT538600.039.346548-49
GTATGCC557000.038.39642746-47
TCGGAAG1194900.038.2582553
ATCGGAA1192950.038.1382062
CGGAAGA1205750.037.8511434
GCCGTCT546300.037.5873450-51
CCGTCTT549900.037.50529552-53
GATCGGA1207800.036.811681
TGCCGTC580050.036.3748150-51
AAGAGCA1256250.036.3548137
CGTCTTC587950.034.5852852-53
Produced by FastQC (version 0.11.5)