FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27631231
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1432160.5183120505923171TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG190600.041.7442244-45
CGTATGC197250.040.6856946-47
TATGCCG201350.039.89203648-49
CTCGTAT192350.038.79613544-45
TCTCGTA206300.036.0464342-43
AATCTCG211250.034.89873540-41
ATCTCGT220800.033.3995742-43
ATGCCGT248850.032.46840348-49
GTATGCC260150.031.1863546-47
GCCGTCT265400.029.68261150-51
CCGTCTT274400.028.95997852-53
TGCCGTC290300.027.75021650-51
ATCGGAA614350.027.672772
AGAGCAC624350.027.6621078
GAGCACA634450.027.4913679
TCGGAAG625500.027.2776643
GATCGGA623600.026.9018251
CGGAAGA652800.026.1444784
CGTCTTC308250.025.51782252-53
GTCACAT347700.024.29586628-29
Produced by FastQC (version 0.11.5)