FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32513780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3313761.0191863265360102TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA866000.049.3823552
TCGGAAG879200.048.791693
GATCGGA860950.048.4936261
AGAGCAC904600.047.452398
GAGCACA916650.046.9320879
CGGAAGA916050.046.8553054
TCGTATG448200.044.29012744-45
CGTATGC458750.043.6284746-47
TATGCCG458150.043.59165648-49
CTCGTAT431500.042.56411744-45
AAGAGCA1031800.041.6855287
TCTCGTA442600.041.04620442-43
AATCTCG445850.040.4918940-41
ATCTCGT458850.039.39069742-43
ATGCCGT515300.038.66028248-49
GAAGAGC1125300.038.434046
GTATGCC527900.037.97653246-47
GCCGTCT528550.036.7108150-51
CCGTCTT543200.035.847352-53
TGCCGTC561100.035.3769350-51
Produced by FastQC (version 0.11.5)