FastQCFastQC Report
Wed 30 Jun 2021
HGMJJDRXY_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMJJDRXY_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917143993
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG103209730.5383514768679141Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA49282630.25706274635574544Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCATATCAATAAGCGGAGGA38037840.19840888393822384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC36129200.18845324155054222Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATATCAATAAGCGGAGGAAA23574610.1229673414520617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG22490970.1173149752033258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21336780.1112946136435564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)