FastQCFastQC Report
Wed 30 Jun 2021
HGMJJDRXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMJJDRXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917143993
Sequences flagged as poor quality0
Sequence length10
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGACGAAC52193343927.22452986868577No Hit
AGACCATCGG44312090823.1135955159316No Hit
AAATCGTTGG40803516921.283490989192487No Hit
AACGAACTGT39348415620.524496722036258No Hit
AGATCTCGGT614455113.2050545615954684Illumina Single End PCR Primer 1 (100% over 10bp)
AAAGGTAGTA124393240.6488466200462387No Hit
ATAGATGCTC121162590.6319952514907418No Hit
AGGTAACACT117237100.6115195333687177No Hit
ATCAGTCTAA97795290.5101092581312435No Hit
GGGGGGGGGG65747910.3429471664103636No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)