FastQCFastQC Report
Wed 30 Jun 2021
HGMJJDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMJJDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917143993
Sequences flagged as poor quality0
Sequence length10
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGGTGAAT52650989027.463241776435517No Hit
TCGGCTCTAC42911271922.38291544958564No Hit
GCCTTCGGTA39785597520.752534835811886No Hit
ACAGTAACTA39541101820.62500362225009No Hit
GGGGGGGGGG678980203.541623386032225No Hit
GTAGCCCTGT118530890.6182680614121195No Hit
GTAACATGCG107059300.5584311892633096No Hit
CACTACGAAA99341010.5181718763051723No Hit
TCCCAAGGGT91440570.47696245213647864No Hit
TCCGAAGGGT31790310.16582119087598443No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)