FastQCFastQC Report
Thu 24 Jun 2021
HGMJ7DRXY_n01_ZmTARGET.20210517-UT4691-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMJ7DRXY_n01_ZmTARGET.20210517-UT4691-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTGGATAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAA13.125No Hit
TGAGGATATAGAGAGGAAGAACAGCTCGTCCGAGACCATGAAGAGCAGCC13.125No Hit
GGGTTCTATAGGGACAGTCGGGGGCATTCTTATTTCATAGTCAGAGGTGA13.125No Hit
GTGATCTATATTCGTTGCCAAGCTTCGAGTGTACTTACTCCTACAATATA13.125No Hit
TATGCCTATAGCGAGCCGGCGCACGGTGGGCGCGTTCGAGGCGTGGCACG13.125No Hit
GACACATATACACTCGGGACGATTTTGCTGCGAGGGCGGTCATTCAAGGG13.125No Hit
CATAGTTATAATTTCCATGACGTTATTTATGAGATGGGTTTTTATGAAAA13.125No Hit
AGTCAATATAAGTAGGCCACCACGCTGCTGCGAAAAAAAAGGCGATGAAA13.125No Hit
GGGGTCGATAGGATGTGGTAGTTGGATATAAACAGTCAAACATAAAGAAT13.125No Hit
AGGAAGTATAGGATGCAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTG13.125No Hit
TTAAAATATACTGCGGCCGCGAGAGCAAGACGAGGCCAGCCTTTTGTGTC13.125No Hit
GTTGGCTTTACTTTCGCGGGGCGGAATCAGAACAAGCGCTGTTGGCTCAG13.125No Hit
TGTTGTTATACTATTTTGCTGTAATTTGCTATTTTTCCTCGTTCTTTGTT13.125No Hit
CGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAAACGGTTGCT13.125No Hit
CATCAATATAAGACAAAAAAAAAAAAAAAAAAAAATAACGGAAAAGCAAA13.125No Hit
GCGGTATATACTTCGTGACTGCGTATTTGCAATACAGGCGTGGGGATCAG13.125No Hit
TGGATTTATGAGGCGCCCTACCCCAACCAACCAATTTACACAGCGGCGGC13.125No Hit
GGCATCTATACACGTCATCAATCGGTAATAGACGACCCTTGTACGAAGCG13.125No Hit
TGGATTTATACTGCGTTGTGATGCTATTGAGTTGACTTAGTGCCTTTCCT13.125No Hit
CGCAATTATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATC13.125No Hit
ACCGAGTATACCAGCTTCTTAAATTAAAACGTAAATAAAAAAGATTTTTT13.125No Hit
TAAGCTTGTATTTGGACACGTTGTCTTTTTGTGTTTAACTTATCTGCTAC13.125No Hit
TCTATGTATAAGAGTCATGAGTTTGTCTACTAGAGTTCTAGTTCCTGTGG13.125No Hit
GAGGAATATAGCCGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGA13.125No Hit
ATGGCATAATAAACTAAGTTTAACCTGAATCTGTTTTAATACTGTTCTTT13.125No Hit
GGGAGCTATACCCGGGTGGCCGGTGTCTTCTTCTGACGTACGGCATGGGA13.125No Hit
TGCAGTTATAGCCTGTCATACCTGTACTCTTTTTTCTATTAGCGTTTGAT13.125No Hit
ATGGGTTATAGCCGAAGACGACGAGGTGGAGGAGGAGTACCATGATGACC13.125No Hit
TGTACATATAGTGGCTGCTTGCAAAGAGGAAACTTTGTGATTCTGTACGA13.125No Hit
CCAGTGGATACGCTCAGTTCTTGTTCATCCAGCCGTGTTAGGAAAACAAA13.125No Hit
GGGGAGTATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACAC13.125Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGCGCTATAGCTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAAT13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)