FastQCFastQC Report
Thu 24 Jun 2021
HGMJ7DRXY_n01_ZmTARGET.20210510-p1107NLSmyc-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMJ7DRXY_n01_ZmTARGET.20210510-p1107NLSmyc-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCGTATAGGTCACATATAGGCGGAAGATCGGAAGAGCACACGTCTGA125.0Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGATTATAGCTCGGGGACTTATATCCAGAAGATCGGAAGAGCACACGT125.0No Hit
GACGTCTATACTGCACTGGTCCTATTGATAACCTTTTTGCACATCTTGCT125.0No Hit
GCTTACTATAGCCAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTAT125.0Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)