FastQCFastQC Report
Tue 10 Oct 2017
HGMHHBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMHHBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286680704
Sequences flagged as poor quality0
Sequence length50
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45630961.591699732954472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1477000.022.3632355
GTTATAC1509800.022.1486113
ATACCGG1446450.021.9107956
GGACCAT889450.021.3549566
TACCGGT1499700.020.6607517
TTATACC1607000.020.5689964
TGTTATA1815600.019.9531692
TGGACCA1054300.019.4660725
GTGTTAT2039150.018.705641
ACCGGTG1671250.018.3310078
GGTATCA464400.017.9199871
GGCTTCA1769300.017.86154614
GGTATTC1469250.017.83890224
TTCAGGG1740600.017.71659317
TGGCTTC1854600.017.42780513
GGGTATT1476300.017.33053623
CCGGTGG1788300.017.1620929
TTAGTCC550200.017.1316978
TGGCATA1428050.017.02939236
GGGGTAT1480150.017.02347422
Produced by FastQC (version 0.11.2)