FastQCFastQC Report
Tue 10 Oct 2017
HGMHHBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMHHBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286680704
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG29432471.0266637966676684No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCAT8981900.013.9062233
CCTACAA3964700.013.8839011
CACCATA8998550.013.8835444
AACACCA9004950.013.8757222
CCTCTCT3423250.013.8533814
CCTTCTT4054950.013.8514971
TCGACTT4723850.013.85073956
CTACTCC4299400.013.8411842
TACTCCA4308450.013.8345763
AGACGTC5349450.013.8313391
TCAGAAC3695200.013.829762
TCAACCG2962900.013.8256971
CGCATCC3709350.013.8245115
TTCCACC2837200.013.8243392
CCATAGC9022550.013.8219856
CTCCAGT4301350.013.8211615
TCCTTAC3468450.013.8187624
AATCCTT3474850.013.8118982
CTCCTAC3031300.013.8115634
AAGCCTT2929200.013.8093171
Produced by FastQC (version 0.11.2)