FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13599211
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG429490.3158197927806253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT14800.028.2315441
ACTCTAG22600.025.2380625
TATAGGG6250.023.5127267
CTAGCAG20300.021.37278
CTGACTC20500.021.3521352
GGGTATA9800.020.7099344
GTATAGG7600.019.335276
GTGCGCA27150.017.94397468
ATAGGGG2952.3646862E-1117.7911078
GGTATAG10050.017.7574635
CTCTAGC26950.017.1367996
TAGCAGA28550.016.7900269
GTGTATA12150.016.704314
TGCGCAT29700.016.6426669
GTATAGT9950.016.1751866
AGGTGCG30400.015.88628266
CTATCGA27650.015.56480517
TCTAGCA47050.015.542487
TCTATCG28600.014.80316716
CTATAGG5250.014.6614846
Produced by FastQC (version 0.11.5)