FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_9-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_9-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20376561
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG415680.20399909484235343No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT209150.10264244295197801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT65450.024.4916061
ACTCTAG99950.019.7146535
GTGCGCA158650.018.99425968
GACTCTA96800.018.259114
GGTGCGC166450.018.23033167
TGCGCAT166450.018.16725269
CTAGCAG89400.017.9312978
AGGTGCG177250.017.15903366
CTGACTC97450.017.0238552
AAGGTGC210000.014.64970465
CCGGGGT13300.014.2103091
GCGCATG220950.014.20881170
CTCTAGC127400.013.8735156
TCTAGCA197500.013.8586637
CTATCGA116700.013.79652417
AGCGCGC62100.013.63901661
GCGCGCC63200.013.45700762
TAGCAGA123050.013.198439
ATGCGCT232650.013.11815456
CGAGCGC65200.013.09789559
Produced by FastQC (version 0.11.5)