FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_8-bZIP3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_8-bZIP3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26137909
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG996930.38141153525325994No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC590120.22577169428510904No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT509420.19489699807279917No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG492530.1884351192744607No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC452890.17326940728120216No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG426250.1630773142564694No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA420260.16078562367020252No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG385730.14757492651764914No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG362470.13867597442473306No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT349370.1336640968487571No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC344770.13190420090604799No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA301310.11527701010819189No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC293830.11241526627091708No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG288180.11025365494998088No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA286960.10978689993908847No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC284610.10888782266400882No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG279110.10678359925424792No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC270430.10346275212757072No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA269670.10317198671094922No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG267310.10226908357512454No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC266780.10206631295563849No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA263280.10072726169488157No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG263270.10072343583413654No Hit
CTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAA261740.1001380791401485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT106950.034.9178281
ACTCTAG148200.028.6704755
GACTCTA140550.027.441914
CTAGCAG140600.026.4625388
CTGACTC145150.025.969372
CTCTAGC174350.022.0818356
GTGCGCA357750.020.73058568
TGACTCT193200.020.3439163
TAGCAGA185350.020.2814279
TGCGCAT366850.020.2735969
GGTGCGC372050.020.04667767
AGGTGCG385400.019.27054266
AAGGTGC426800.017.49148665
CTATCGA208750.017.47141517
TCTATCG214500.017.0683716
TCTAGCA364750.016.2842437
AGCGCGC135600.016.15752861
GATCTAT232450.015.81074714
GCGCGCC139250.015.73400962
GCAGATC238500.015.64437211
Produced by FastQC (version 0.11.5)