FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_7-HHO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_7-HHO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17903356
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA237980.13292479912704636No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT206220.11518510831153668No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG184720.10317618663227163No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT181650.10146142432737192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG68800.047.36404811
ACGTCTG73650.044.10245515
ACACGTC78500.041.46845213
CACACGT79700.041.19531612
AGCACAC81800.040.1363910
CACGTCT83850.038.9465114
TATGCCG40100.037.96695346
GTATGCC42250.037.4431745
CGTATGC41300.035.84686744
GAGCACA94200.035.224319
CGTCTGA92750.035.1336516
CAGTCAC96450.033.38620827
AGAGCAC99150.033.2892578
TCCAGTC99550.032.5926725
CCAGTCA101650.031.91933626
AGTCACC103050.031.28041528
GTCACCA114850.028.18847729
TCGGAAG116700.027.951553
CCAGATC117400.027.27808433
CTCGTAT48350.027.2177542
Produced by FastQC (version 0.11.5)