FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_6-HSFB2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_6-HSFB2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24814075
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG588940.2373411058038633No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC321710.12964819361592161No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT302840.12204363853982064No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG277190.11170676319790279No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC264060.10641541141469106No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG250330.1008822613778672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT87900.027.1554491
ACTCTAG128400.022.215445
GACTCTA119600.021.3918614
CTAGCAG112950.020.9479358
GTGCGCA213600.019.49865268
CTGACTC124300.019.2595252
GGTGCGC225000.018.65071767
TGCGCAT224750.018.6403269
AGGTGCG237700.017.63951166
TAGCAGA152050.015.8834629
CTATCGA151850.015.58196617
CTCTAGC162400.015.4308326
TGACTCT172450.014.9373473
AAGGTGC284200.014.88884865
TCTATCG158500.014.79595216
GCACACG36200.014.60031511
AGCGCGC88900.014.36977561
GCGCATG302700.014.27951370
GCGCGCC91050.014.26109362
TCTAGCA264900.014.1642497
Produced by FastQC (version 0.11.5)