FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_5-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_5-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13761948
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG172700.12549095520488815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG57700.018.8039995
GCTGACT42050.017.728681
GTGCGCA72250.017.2453468
GACTCTA51500.016.1066594
GGTGCGC78750.015.91080267
CTAGCAG47600.015.8830838
TGCGCAT78850.015.80184969
AGGTGCG85700.014.66132666
GCACACG20950.014.20133811
GTATGCC20350.013.58696145
TATGCCG19650.013.53663346
CGTATGC21500.013.51136644
CCGGGGT9650.013.4195261
CTGACTC59350.012.9147562
GCGCATG101850.012.78325770
GTCACGC24450.012.59689229
TCTAGCA103150.012.4531317
TCACGCC24850.011.68991430
AAGGTGC109250.011.62906565
CTAGTTG30800.011.4777998
Produced by FastQC (version 0.11.5)