FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_4-bZIP3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_4-bZIP3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22808053
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG854140.3744905363031206No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC440540.19315107694637504No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT425830.1867016005267964No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG409200.17941031617209938No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC376310.1649899708668688No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG371180.16274076529022447No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG323250.14172625782656678No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA315000.1381091143553551No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG311510.13657895305662435No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT304230.13338709796930057No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC291690.12788903989305883No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG256730.11256112040777877No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA253060.11095203961513067No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC247840.10866337429152764No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG246500.10807586250347628No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA243960.10696222075597596No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC243630.1068175350171275No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG242650.10638786221691084No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC234780.10293732656619134No Hit
AGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGATGAGG231920.10168338349617129No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA230210.1009336483039565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT94700.032.8559271
ACTCTAG128350.027.2419515
GACTCTA122600.026.9208564
CTAGCAG123050.025.3162788
CTGACTC129000.024.4996552
CTCTAGC154850.020.5909216
TGACTCT163400.020.3485073
TAGCAGA157550.019.6838749
GTGCGCA311900.019.32310168
TGCGCAT319550.018.8605169
GGTGCGC320300.018.86005467
AGGTGCG331650.018.23571666
CTATCGA174800.017.58084717
TCTATCG181900.016.97159216
AGCGCGC121350.016.61275961
AAGGTGC369600.016.47693465
GCGCGCC124850.016.25917662
TCTAGCA315450.015.9889587
CGAGCGC131450.015.38956559
GATCTAT199700.015.31883714
Produced by FastQC (version 0.11.5)