FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_3-HHO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_3-HHO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20665928
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA257920.12480446075298433No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT209460.10135523553551527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT72300.022.800631
ACTCTAG99450.020.3769955
CTAGCAG88550.019.4075578
GACTCTA92400.018.6362924
CTGACTC101500.016.6205442
CTCTAGC123700.014.5432286
CTATCGA113300.014.27234217
TCTATCG119250.013.76567916
TAGCAGA126200.013.5068429
TCTAGCA196600.013.2988797
TGACTCT141600.012.7788443
GCGGGCT17600.012.52831
CTAGCAT131000.012.10315226
GATCTAT135850.011.98068814
GGACAGC135600.011.53745540
ATCTATC146800.011.44470615
CGGGAAT173600.011.2700621
ATTCTAG142700.011.08636723
TTCTAGC147400.010.92274924
CGGCAAT33150.010.7691371
Produced by FastQC (version 0.11.5)