FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_23-HHO3-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_23-HHO3-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17479462
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA265170.1517037538111871No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT251700.14399756697317115No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT221040.12645698134187427No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG194820.11145651965718396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT63000.026.7218511
ACTCTAG98500.025.9035635
CTAGCAG75850.022.658328
GACTCTA87900.021.0635974
CTGACTC87300.019.8851762
CTAGTTG47200.017.5755188
CTCTAGC112350.016.1994766
TAGCAGA109400.015.9657749
TACTCTA53800.015.8735224
TGACTCT125300.015.1952753
GGACACC49250.015.06560729
CTATCGA112200.015.03604317
TCTAGTT61800.014.7826597
TCTATCG116850.014.52784216
TCTAGCA188350.014.42104157
AGGACTG57650.013.41683362
CTAGCAT125550.013.24223426
GACACCT56850.013.23625230
CTAATGC58450.013.23399421
CGGGAAT164800.013.0823421
Produced by FastQC (version 0.11.5)