FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_22-HSFB2A-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_22-HSFB2A-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19545555
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG234410.11993008128958221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG95000.023.4314335
GTGCGCA89750.019.06922568
GCTGACT63850.018.637221
CTAGTTG55900.018.4087478
TACTCTA58750.018.110484
TGCGCAT98050.017.56208869
GGTGCGC99050.017.3494567
CTAGCAG69600.016.9476458
AGGTGCG108350.015.69878566
GGACACC58600.015.64812829
GACTCTA82250.015.1913574
AGGACTG65400.014.50274662
TCTAGTT77050.014.4003527
CTGACTC82900.014.2700062
CTAATGC68450.014.21528521
ACCTCTA71350.013.8829317
GGACTGT68350.013.77439163
GACACCT69650.013.56754730
CCTACAG84800.013.53744343
GCGCATG132800.013.282845570
Produced by FastQC (version 0.11.5)