FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_21-EV-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_21-EV-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842866
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG541220.32133486070601047No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC377610.22419581085546844No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT316710.18803806905546835No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG265110.15740195285054218No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG253480.1504969522407885No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA250510.14873359438945843No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC236580.1404630304604929No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG211240.12541808502187218No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC203360.120739546345616No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG182890.10858603280463075No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA171650.1019125842359608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT69250.035.0752371
ACTCTAG105250.029.7289035
CTAGCAG90500.026.5317618
GACTCTA96750.026.4803434
CTGACTC95050.025.885952
CTCTAGC119050.021.2262786
TAGCAGA117350.020.8191459
TGACTCT125800.020.2818013
GTGCGCA187300.019.8634768
CTATCGA123500.019.61188517
TGCGCAT192250.019.4248569
GGTGCGC195150.019.01064967
TCTATCG128200.019.00226216
AGGTGCG204800.018.16615166
CTAGTTG39650.018.0085018
GATCTAT139550.017.10551314
ATCTATC143550.016.79954715
GCAGATC149250.016.29924411
AAGGTGC234350.016.0099365
TAGCATG147950.015.96882327
Produced by FastQC (version 0.11.5)