FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_2-HSFB2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_2-HSFB2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18943125
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG234590.12383912369263254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA97400.018.68538368
GCTGACT56450.018.0422731
GGTGCGC104650.017.55810767
TGCGCAT104900.017.4161769
ACTCTAG79500.016.6853545
AGGTGCG114500.016.10878466
CTAGCAG70000.015.9508388
GACTCTA78200.015.1724714
GCGCATG137500.013.66876370
AGCGCGC36800.013.41000561
GCGCGCC37900.013.11314262
CTGACTC82200.012.9438662
AAGGTGC147950.012.56137565
ATGCGCT146600.012.48605856
CGAGCGC42600.012.07717559
TCTAGCA145850.011.9272327
TCATGCG155100.011.91460854
GCGCTTC155450.011.91029758
CTATCGA87650.011.82023517
TGCGCTT161550.011.43890957
Produced by FastQC (version 0.11.5)