FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_19-HHO3-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_19-HHO3-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18596765
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA292440.15725315666461342No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT254660.13693779536387107No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT239550.1288127262994397No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG215030.11562763738747035No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA192620.10357715441368433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT71350.027.5187361
ACTCTAG102850.027.2920115
CTAGCAG85950.023.2120918
GACTCTA91050.022.3336284
CTGACTC97250.020.4057732
CTCTAGC119400.017.3826436
CTAGTTG48850.017.052888
TAGCAGA119700.016.6674619
TACTCTA52250.016.5452654
CTATCGA117850.016.45390517
TCTATCG120050.016.1815316
TGACTCT133300.015.8323993
GGACACC48000.015.312201529
GCGCCCT10200.015.0977471
TCTAGTT63100.015.0321317
TCTAGCA205300.014.8323837
GATCTAT138700.013.95546914
ATCTATC140100.013.89085415
CTAGCAT136200.013.79999526
GACACCT54700.013.75658830
Produced by FastQC (version 0.11.5)