FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_18-HSFB2A-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_18-HSFB2A-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12491868
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG146730.11746041504761338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG62650.022.9049995
GCTGACT37900.019.9470441
CTAGTTG37000.018.2575388
TACTCTA40550.017.6077274
GTGCGCA58700.017.41026368
CTAGCAG44700.016.756878
TGCGCAT63500.016.20444369
GGTGCGC64550.016.15773867
TCTAGTT48900.015.6754547
GGACACC39900.015.4383329
GACTCTA51900.015.2407064
AGGACTG44350.014.99410362
AGGTGCG69900.014.77084666
CTAATGC45150.014.65167221
CTGACTC52000.014.4710212
GACACCT44700.014.40691130
GTACTCT51250.014.1363983
CTCTAGT55700.013.8868776
CCTACAG62300.013.70762843
ACCTCTA48300.013.62360817
Produced by FastQC (version 0.11.5)