FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_17-EV-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_17-EV-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18465873
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG525090.28435698653402414No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT303790.16451429076762306No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC298170.16147083866546685No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG237850.12880517482168322No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG231050.12512270608597817No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA229880.12448910484762893No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC225840.12230128518700416No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG199610.10809670357854188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT72150.032.1130521
ACTCTAG112250.027.1580645
GACTCTA100200.024.3113174
CTGACTC98700.023.6165812
CTAGCAG98650.023.2041958
GTGCGCA188650.021.13133268
TGCGCAT198050.020.18139569
GGTGCGC198400.020.0575967
AGGTGCG208000.019.09820266
CTCTAGC127950.018.8198576
TAGCAGA125150.018.4866739
TGACTCT133550.018.3710543
AAGGTGC237500.016.85863365
CTATCGA133700.016.83360517
TCTATCG138600.016.39009316
CTAGTTG42700.016.1482018
TCTAGCA236800.015.1800167
GCGCATG277300.014.94382570
CGAGCGC79450.014.88958959
ATCTATC154350.014.87645615
Produced by FastQC (version 0.11.5)