FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_16-bZIP3-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_16-bZIP3-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12374807
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG433270.35012263221559736No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT234840.1897726566563826No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC221370.17888763840922933No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG198170.16013987127233578No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA190450.15390139013885226No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG178700.14440629255874454No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC165560.13378794513724537No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG146010.11798971895076828No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA140370.11343207211231658No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT137570.11116941056131219No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA129000.10424405002841661No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC127590.10310463831880368No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG127260.10283796749314959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT51100.032.602191
ACTCTAG66850.028.4816555
GACTCTA67300.025.950844
CTAGCAG64950.025.9209658
CTGACTC72200.023.6076322
GTGCGCA146050.021.71137468
GGTGCGC151200.020.99501467
TGCGCAT152050.020.90066569
CTCTAGC85400.020.5737556
AGGTGCG158750.020.04060466
TAGCAGA85650.019.779039
TGACTCT93100.019.2477953
AAGGTGC178000.017.99126865
CCGGGGT7000.017.4997021
CTATCGA98750.016.9778217
TCTAGCA170000.016.5330097
TCTATCG101450.016.42266516
GCGCATG205150.016.00265370
TCAAGGT207700.015.67137663
GATCTAT110550.015.22912914
Produced by FastQC (version 0.11.5)