FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_15-HHO3-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_15-HHO3-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19148842
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA256920.1341699931515441No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT207670.1084504222239653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT64350.022.8980161
ACTCTAG93150.020.3647755
CTAGCAG78050.019.3282478
GACTCTA86900.018.3254594
CTGACTC93350.016.4218852
CTCTAGC115700.014.0663586
TCTAGCA181950.013.6388157
TAGCAGA113500.013.5690169
CTATCGA107800.013.53969417
TGACTCT129400.012.8205343
TCTATCG113600.012.78684916
CGGGAAT159200.012.399391
CGGGGGT13000.011.5768241
CTAGCAT123350.011.49201226
GATCTAT129450.011.383656514
CTTACAG144700.011.0779132
GCGGGAC16800.011.0415721
CATGCCG147550.010.9587663
GGACAGC126150.010.93119340
ATCTATC134250.010.82009415
Produced by FastQC (version 0.11.5)