FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_14-HSFB2A-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_14-HSFB2A-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16474709
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG185080.112341893261969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG82600.023.7711075
CTAGTTG48150.018.9002428
GTGCGCA74800.018.6694468
GCTGACT52400.018.434831
CTAGCAG56700.017.840428
TGCGCAT81300.017.26290369
TACTCTA53450.017.0905784
GGTGCGC83800.016.78966567
GACTCTA68600.016.0712764
GGACACC51850.015.72777129
AGGTGCG92700.015.40424466
TCTAGTT65100.015.323187
CTGACTC72050.014.2329072
CTAATGC58550.014.22753921
CTCTAGT69550.014.1912256
GACACCT60050.014.04636830
ACCTCTA63300.013.76856217
GTACTCT66650.013.3905993
CCTACAG75900.013.37261243
CCGGGGT12400.013.2659231
Produced by FastQC (version 0.11.5)