FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_13-EV-MS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_13-EV-MS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24535910
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG697410.2842405274554724No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC368110.15002907982626282No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT354690.1445595455803351No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG329310.13421552328811118No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC303150.12355359960156359No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG301340.12281590534037662No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG256590.10457733175578163No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA255800.10425535470255638No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG248970.1014716796727735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT89950.030.3498881
ACTCTAG129300.024.9031265
GACTCTA127000.023.397454
CTAGCAG119950.023.1983038
CTGACTC128200.021.64952
GTGCGCA250100.020.12358368
TGCGCAT259350.019.44633769
GGTGCGC262300.019.21428967
AGGTGCG273650.018.44292866
CTCTAGC161700.018.1167016
TAGCAGA158950.017.6605439
TGACTCT179100.016.7278463
CTATCGA166950.016.22694417
AAGGTGC314250.016.16040465
TCTATCG178050.015.31364516
TCTAGCA296900.014.8894597
GCGCATG351500.014.88592970
TCAAGGT369400.013.9940663
ATCTATC197050.013.85483315
CCGGGGT15000.013.7665261
Produced by FastQC (version 0.11.5)