FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_12-bZIP3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_12-bZIP3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17542484
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG676590.3856865424531668No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC358380.20429261899284046No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT335910.19148371462103086No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG312830.17832708298323088No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC295520.1684596092548809No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG289070.16478282095055638No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA264800.15094783612172596No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG251520.14337764252770588No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG248570.14169601066758847No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT228120.13003859658643555No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC220720.12582026581868339No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA197430.11254392479423098No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG189280.10789806050272013No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC189180.10784105603288566No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG186970.10658125724954345No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA185150.10554377589855575No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC183090.10436948381996529No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG181520.10347451364356383No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC178750.10189548982914849No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA176420.10056728568200489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT75600.033.7496641
ACTCTAG103500.027.3574475
CTAGCAG96050.026.2743858
CTGACTC99200.026.143892
GACTCTA100050.026.0968384
CTCTAGC122800.021.5187596
GTGCGCA245450.020.39068268
TAGCAGA125700.020.3831679
TGCGCAT249900.020.06959769
GGTGCGC251450.019.94588367
TGACTCT138650.019.3362663
AGGTGCG261250.019.18427866
CTATCGA143550.017.55518317
AAGGTGC292000.017.34380765
TCTATCG147750.017.10377316
TCTAGCA255650.016.0874777
AGCAGAT167600.015.64267910
ATCTATC162850.015.58237815
GCAGATC166050.015.55668611
TCAAGGT329350.015.45131763
Produced by FastQC (version 0.11.5)