FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_11-HHO3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_11-HHO3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17052306
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA238430.1398227313068391No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT191070.11204936153503227No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG182630.10709988432063089No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT178850.1048831753312426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55900.023.9174611
ACTCTAG81300.020.3198915
GACTCTA75300.019.0107044
CTAGCAG70550.018.8028458
CTGACTC82400.016.0981352
CTCTAGC103800.013.588696
TCTAGCA164050.013.4200227
TAGCAGA102050.013.3420649
CTATCGA97050.013.2358917
TCTATCG102050.012.6904716
CGGGAAT150000.012.4598711
TGACTCT118450.012.4396833
CGGGCAC11350.012.0263071
CTTACAG131400.011.852962532
CGGGGAT29750.011.4116461
CTAGCAT111200.011.29964226
CATGCCG136100.011.23787863
GATCTAT117750.011.11750414
ATCTATC118950.011.09356115
TTACAGC142250.011.07190933
Produced by FastQC (version 0.11.5)