FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_10-HSFB2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_10-HSFB2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18902315
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG248100.13125376441986072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA93500.020.02638668
TGCGCAT100650.018.53419969
GCTGACT59550.018.4549481
GGTGCGC102550.018.4297167
ACTCTAG82650.018.4209235
AGGTGCG111350.016.8474866
CTAGCAG68750.015.9860188
GACTCTA76600.015.85497954
GCGCATG135250.014.49142670
AAGGTGC141900.013.44232865
CTGACTC83450.013.2533532
ATGCGCT145450.012.99392756
AGCGCGC37950.012.81927461
GCGCGCC38750.012.73525962
CTATCGA87550.012.55343517
GCGCTTC153850.012.32997558
TAGCATG82250.012.29814327
TCTAGCA146400.012.240747
TCATGCG154350.012.19933154
TCTATCG94450.011.74758816
Produced by FastQC (version 0.11.5)