FastQCFastQC Report
Tue 15 Jan 2019
HGMG7BGX9_n01_1-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMG7BGX9_n01_1-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21477862
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG401430.1869040782550889No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT237290.1104812015274146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT74800.025.968781
ACTCTAG106100.021.1780955
GACTCTA102400.020.5418114
CTAGCAG99050.019.647848
GTGCGCA157900.019.4613368
GGTGCGC165250.018.59572867
TGCGCAT166450.018.5037269
CTGACTC108550.018.4105362
AGGTGCG174900.017.68978766
CTCTAGC133050.015.7835116
TAGCAGA132450.015.2481939
CTATCGA129550.014.88632417
AAGGTGC212700.014.66122865
TCTAGCA224100.014.6348827
TCTATCG134300.014.41207416
GCGCATG220850.014.37374870
TGACTCT152050.014.1102343
ATGCGCT228850.013.51952756
CGAGCGC64400.013.26062859
GCGCTTC233500.013.19033858
Produced by FastQC (version 0.11.5)