FastQCFastQC Report
Fri 4 Nov 2016
HGM2WBGXY_n02_grads_set4_dna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGM2WBGXY_n02_grads_set4_dna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55876525
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGC4988540.8927792127373704No Hit
TTGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG4876980.8728137621299821No Hit
TTGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCA3609750.6460226365186453No Hit
TTGAGTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTT2718040.48643683550471334No Hit
TTGAGTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCAT2636590.47186005214175364No Hit
TTGAGTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGA2156220.38589013901634006No Hit
TTGAGTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT2097370.37535798799227404No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCACATAC2007570.359286838256316No Hit
TTGAGTCCATTCTATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT1754160.31393505591122567No Hit
TTGAGTATCACAGCCAAAGGCATTAGAAAGGCTTGAAATCCAAGGAAAGA1682580.3011246672909598No Hit
TTGAGTTTAAACTCCAAGTTAGATCAAACTAGAAAGACTTTTGAAGCAAA1536060.2749025641805749No Hit
TTGAGTCCAACCAATGATTCCATTCATTTCCATTCAATGATTCCATTCGA1504230.26920607535991187No Hit
TGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGCC1291550.2311435795264648No Hit
TGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGGG1288930.23067468852080547No Hit
TCAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGA1253940.22441266703682808No Hit
TTGAGTTGGAACGCATCGAATGCACTCCAATGGAATCAACATCGAATGGA1244300.22268743448165396No Hit
TTGAGTAGCATCATCATCTAATGGAATCGAATGGAATCATCGAATGGACT1214690.2173882502535725No Hit
TGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCAG1184200.21193157591671996No Hit
TTCAGGTCCTTGCTGACAATCACCCGATTAGTAGCCACCCGTCGCTCATA1064140.1904449140314291No Hit
TCAAGAATTAATTGAAGACGTTCTTCCTAAGAAAGCTAAAGATCAATTGA1060540.18980063631372923No Hit
TGAGTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTTT1001720.17927385427064407No Hit
CAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGAC916070.16394541357036788No Hit
TTGAGTATCACAGTAGTCCTCTGTGTACCTACTGGCCCAGAATGCAGGAG871760.15601542866167858No Hit
TGAGTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCATC861780.15422934765538837No Hit
TTGAGTCTAGCTGGTCGAGTAGATGAGGCGACAGAGTGAATAGAGCAAGG822030.1471154478557856No Hit
TGAGTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATTC789420.1412793655296209No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750370.1342907419529042No Hit
TTGAGTTACAGACCGAAGAAGAAGCAATAAAACCCTGAATCACATCAAGA746010.13351045005035655No Hit
TGAGTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGAG741740.13274626509075144No Hit
TTGAGTCCATTCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT721930.1292009479830752No Hit
TTGAGTCCTAGCTGGGCACGAAATAATAACCTTCACGATCTACTCACACA702510.12572542762814976No Hit
TGAGTCCATTCTATGATTGTTCCATTCGATTCTATTCGGTGATTCCATTC688810.12327359298023634No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCTCTTAC683740.12236623519447568No Hit
TTGAGTAGACGACTGTCCATCACTGCCGGGTGTAATCTTCGATGCGATCC678470.12142308420217612No Hit
TGAAGTGGATCAGGGGAGTGTGGCGAGAGACGGCGGTGGCAGGCTATCAG663120.11867595560031693No Hit
TTGAGTCAGAGCGTGCCAGTACTTTGATTGCCAGGGAGTCTGTCCAAATA641740.11484966182131048No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCTCATAC633170.11331592292111937No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCTCTGAC605900.10843551920954282No Hit
TCAAGTTCATCTGCTGTGAGATATTGTGGCATTTCTTCTGTGTTATACTT603020.10792009703538291No Hit
TCCAGCCTGGGTGACACAGCAAGACCTTGTCTCCAAAAAATAATAAAATA599480.10728655727964471No Hit
TTGAGTCTCAGCACTTTGATTCCATTCCATTCGAGTCCGTTCCATTCCAG586020.10487767447957796No Hit
TTGAGTCTTCCGAATTGTCCTGCAGGTTGCGCAACACTTAATTTCCCGGT565250.10116054998051506No Hit
TTGAGTTCCAAATTCTGCCACCCCAAATCCTACAAATATGCACATTAAAG564900.10109791186907204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA9490850.0136.536041
TTGAGTT11629050.0135.266621
TTGAGTC7992450.0132.754431
CGCCTTA926300.0126.134673
CCGCCTT948550.0123.623552
GCCGCCT835700.0123.352731
GCCTTAC987300.0118.803364
TTGAGTG3871150.0114.71971
TGAGTAT5313050.0105.516182
TACACTG1112000.0104.965948
AGTATCG1234850.0104.210784
GAGTATC2583150.0103.7769553
TGAGTTC5042050.0102.6574552
TATCGAG767750.0101.351946
TGAGTTT4132700.0100.373622
TGAGTTA3445450.0100.07132
TTGAGAG1240150.0100.040381
TGAGTCT3365600.098.877612
TGAGTCC6294500.098.830372
TGAGTAC1938300.098.483432
Produced by FastQC (version 0.11.2)