FastQCFastQC Report
Fri 4 Nov 2016
HGM2WBGXY_n02_grads_set4_cdna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGM2WBGXY_n02_grads_set4_cdna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53490762
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTT17162603.20851664068648No Hit
TGAGTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTTC9324661.743228111052148No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGG6614681.2366023127507513No Hit
TTGAGTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTA4289450.801904822369141No Hit
TTGAGTGATAAACTAATACTTTGTTATAATTTGATATTGTATCCACAAAA2873140.5371282615117728No Hit
TGGAGTGGAATGGAATGGAGTGGAGTGCAATGGAATGGAATGGAATGGAA2655130.4963716912464249No Hit
TGAGTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTAC2002710.37440296700204045No Hit
TTAAGCCTAAAAATAAGGCAAAGGTAAGTATTTTACTAACTTCTAATTTT1898920.3549996165693059No Hit
TTGAGTATCACAGGTAGATTCTGGATATTAGCCTTTTGTCAGATGGATAG1569870.2934843216479137No Hit
TTGAGTATAAGCAGCCCTTATTCCTGAAGATTTGACAGGAAATCATATTT1552150.29017160009797577No Hit
TTGAGTATTACAGCCATTTGATCGTTGACAAACCTGACAAAAACAAGCAA1512080.28268058697686904No Hit
TGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGGG1347510.25191452684857996No Hit
TTGAGTTAATTTTTGTATAAGGTGTAAGGAAGTGGTCCAGTTTCAGTTTT1294880.2420754447281944No Hit
TTGAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAG1269900.23740547947325932No Hit
TTGAGTTGACTACCACACGTCACAAACCAGTTTCTGAGAATGCTTCTGTC1198010.22396577562308795No Hit
TTGAGTTCGTCAAACCGCTTAAAAAAGATTAGGGATGATGCTGAATCTAA1056510.19751260974745508No Hit
TTGAGTCCTCATTTGATTTGGCTCTTTTCAACTTTTGTTTCCTAATCTTA1043060.19499815687800448No Hit
TTGAGTATCACAGGGGAACCAGGGACGTAAGGCAGACGCCACAGCCCTGA1011050.18901394599688073No Hit
TTGAGTATCACAGGGAGACCAAGAAACTACCACATACAACATGTCCTGAA1002090.18733889040503854No Hit
TTGAGTTTGCGAGAGGGTTAAGAGGAATCTTGTTGTTAATGCTCAGGATA978740.18297365066513727No Hit
TTGAGTATGCCAGAGTTAGAAGTATCTTTTATCTCAGCAGTCTTAGCTAA961190.17969271030388387No Hit
TTGAGTATCACAGGCCTGATTGACCTGGATGATTCTATTAAATACATAAA866490.16198871872492676No Hit
TTGAGTGTATGTGTGTGTGTTTTCTGTAGCATATGTTTATTTCTTCTTGT821630.15360222387559183No Hit
TCAAGATGGATTAAAGACTTACATCTTTGACCTAAAACCATAAAAACCCT703430.13150495033142356No Hit
TTAAGCTGGATCTTTTTTTGGGTAGCAGCCAGTTTAACTTGATGATAAAC680640.12724440156601247No Hit
TTGAGTTGCACTAGACAGAAGCATTCTCAGAAACTTATTTGTGATGTGTG666920.12467947269100411No Hit
TGGAGTGGAATGGAATGGAGTGGAGTGGAATGGAATGGAATGGAATGGAA655740.122589392164576No Hit
TCAAGGAGGAGCTAGATAGGGAATATGTTGGGTTTCCCTGAATAAATCAG629040.11759787605942125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG608610.11377852497221858No Hit
TTGAGTTATTTCAATAATGAGAATCTTTGCGACCGACAAGCATTTATTGT602880.11270731196538197No Hit
TTGAGTATCACAGCCCCCCAAATAAGTGTGGAATAAGTGATTAGAATATC543490.10160446022436546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA12770800.0137.412811
TTGAGTT11707900.0132.459151
TTGAGTC5186900.0129.503251
TTGAGTG7033900.0121.469661
GAGTATC2379850.0115.1800843
AGTATCG798300.0112.283064
TGAGTAT6250500.0108.7073442
TGAGTTC3266250.0108.6520842
TGAGTTA6120700.0108.400722
GAGTCCT1578400.0107.524793
CCGCCTT382250.0106.839992
GAGTTAC1844300.0106.643283
TGAGTAC2733050.0106.187662
GTATCGC388650.0105.305425
TGAGTCC3149700.0105.136832
CGCCTTA388600.0104.6417853
TGAGTCT2253850.0104.415012
GTATCGG337900.0104.387875
CTTCCGA539450.0104.172427
GAGTTCC1123250.0104.0187453
Produced by FastQC (version 0.11.2)