FastQCFastQC Report
Fri 4 Nov 2016
HGM2WBGXY_n01_grads_set4_dna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGM2WBGXY_n01_grads_set4_dna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55876525
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGANTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCAT1739560.3113221518338873No Hit
TTGANAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG1604650.28717784436308447No Hit
TTGANTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTT1503840.26913627860716105No Hit
TTGANTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGA1239290.221790814657855No Hit
TTGANTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGC1193040.21351363564573855No Hit
TTGANTAGCATCATCATCTAATGGAATCGAATGGAATCATCGAATGGACT965050.17271116985174007No Hit
TTGANTCCATTCTATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT949800.16998193785315033No Hit
TTGANTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCTCTGAC930770.16657621425097568No Hit
TTGANTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT923010.16518743783726708No Hit
GTGANAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG835400.14950822371290984No Hit
TTGANTTGGAACGCATCGAATGCACTCCAATGGAATCAACATCGAATGGA817900.1463763181407577No Hit
TTGANTCCAACCAATGATTCCATTCATTTCCATTCAATGATTCCATTCGA775730.13882932054203442No Hit
GTGANTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGC761110.13621283714404217No Hit
GTGANTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCA705330.126230111840348No Hit
TTGANTCTAGCTGGTCGAGTAGATGAGGCGACAGAGTGCTTAGTGGATGG682360.12211926206935739No Hit
CAAGNCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGAC648840.11612032065344079No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA3511750.0234.407351
TTGAGTT4570750.0228.614041
TTGAGTC3098800.0215.871321
CGCCTTA523400.0210.660433
CCGCCTT547000.0200.580442
GCCGCCT532500.0199.743711
TGAGTAT1864300.0192.617262
GCCTTAC707300.0191.612094
AGTATCG542950.0183.018074
GAGTATC1029800.0179.76423
TGAGTTC2058400.0175.710222
TGAGTTA1414600.0172.337112
TGAGTCT1374650.0170.540862
GAGTACG282200.0167.64743
TGAGTAG2003200.0165.712192
GTCCTAG542650.0164.095785
TGAGTCC2331450.0163.306992
GTGACCG266350.0161.674385
TGAGTTT1744600.0161.56582
TGAGTAC807550.0161.277452
Produced by FastQC (version 0.11.2)