FastQCFastQC Report
Fri 4 Nov 2016
HGM2WBGXY_n01_grads_set4_cdna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGM2WBGXY_n01_grads_set4_cdna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53490762
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGANTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTT9669631.8077196208197595No Hit
TGAGNAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTTC4884890.9132212399591542No Hit
TTGANTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGG4228070.790429943772347No Hit
TTGANTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTA2411040.4507395127405364No Hit
TGGANTGGAATGGAATGGAGTGGAGTGCAATGGAATGGAATGGAATGGAA1768910.3306944851524082No Hit
TTGANTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAG1403790.26243596978483874No Hit
TGAGNGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTAC1251930.2340460208811383No Hit
TTGANTGATAAACTAATACTTTGTTATAATTTGATATTGTATCCACAAAA1096220.2049363215278182No Hit
TTAANCCTAAAAATAAGGCAAAGGTAAGTATTTTACTAACTTCTAATTTT981430.1834765412390274No Hit
TTGANTATTACAGCCATTTGATCGTTGACAAACCTGACAAAAACAAGCAA836700.1564195327783889No Hit
TGAGNGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGGG790780.14783487287019767No Hit
TTGANTTAATTTTTGTATAAGGTGTAAGGAAGTGGTCCAGTTTCAGTTTT779020.14563636240590477No Hit
GTGANTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGG576900.10785039854171455No Hit
TTGANTTGCACTAGACAGAAGCATTCTCAGAAACTTATTTGTGATGTGTG545970.10206809168282178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA5070200.0239.213321
TTGAGTT4969600.0222.951751
TTGAGTC2184700.0205.699831
GAGTATC965750.0201.575013
TGAGTTA2562450.0195.522662
AGTATCG376650.0193.736074
TGAGTAC1156300.0185.34932
TGAGTAT2630650.0184.442922
GAGTTAC826750.0182.67253
TGAGTTC1382900.0181.977372
TTGAGTG3114400.0178.470631
GTATCGC191050.0177.513995
TGAGTAG2825850.0171.474952
GAGTCCT737400.0171.24553
GAGGTTC491050.0171.227775
GTACGTC203550.0169.072835
TGAGTCT1004450.0167.66722
TGAGTCC1381250.0167.234482
GAGTTCC516800.0165.21713
CCGCCTT279200.0162.462022
Produced by FastQC (version 0.11.2)