FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6384092
Sequences flagged as poor quality0
Sequence length116
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5707888.940785941054735No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147900.2316695937339249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCT824850.0107.8174442
ATAGCTC826700.0107.410943
GCATAGC829500.0106.801211
CATGTTA147200.0105.457762
TAGCTCT840950.0105.3811654
ACCCGTC105150.0105.354391
ACATGTT148700.0105.15161
ATGTTAC147400.0104.842073
GGACCAT132800.0104.824472
GTTACCG141800.0104.804645
CCCGTCC119950.0104.724052
TGTTACC144500.0104.456614
TTAGAGT138800.0104.120973
ACGGAAC260350.0104.023831
AGGACCA135250.0103.3062741
TAATGCG72650.0103.211615
GAGTCTG148050.0102.892446
ACCTAGT98900.0102.6848459
TCACCTA100150.0102.677917
CTTAAAC863850.0102.115599
Produced by FastQC (version 0.11.5)