FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA4_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA4_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466408
Sequences flagged as poor quality0
Sequence length116
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT851182.455510141910589No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT177350.5116247135363178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108420.3127733377028901No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80630.232603894290574No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36980.10668103696968158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT114250.085.817151
AGCAGTG120500.081.249892
GTATCAA144600.068.3361059
TGGTATC145850.067.298017
AGTGGTA146200.067.1533665
CAGTGGT145850.067.0137944
GGTATCA147350.066.836838
GTGGTAT156300.062.8689166
GCAGTGG160200.061.1950263
ATCAACG139200.039.02906410-11
CAACGCA142900.038.0168712-13
ACGCAGA144100.037.56672714-15
AGAGTAC143050.037.53496618-19
GCAGAGT150150.036.0164316-17
TCAACGC141800.034.78306612-13
AGTACTT154900.034.4505220-21
AACGCAG144250.034.07793814-15
TATCAAC146900.033.65180210-11
GTACTTT148550.032.68433822-23
GAGTACT150400.032.373720-21
Produced by FastQC (version 0.11.5)