FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA4_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA4_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21038
Sequences flagged as poor quality0
Sequence length116
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6062.880501948854454No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.4753303545964445No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT480.22815857020629338No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1045726780112178No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1045726780112178No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCC153.652939E-4109.928588
TATCCCA153.652939E-4109.928588
CTATCCC600.0109.928587
CGATAAT450.0109.928572
ACATGTT750.0109.928571
TTACCGT500.0109.928576
GCGACCC208.252795E-6109.928579
GGACCAT700.0109.928572
TGCGACC208.252795E-6109.928578
TAATGCG251.8705941E-7109.928575
TACCGTC401.8189894E-12109.928577
TATCCCC450.0109.928578
CCATCCA650.0109.928575
ATGTTAC700.0109.928573
AATGCGA208.252795E-6109.928576
TACTATC800.0109.928575
CATGTTA750.0109.928572
ACTATCC800.0109.928576
ACGGAAC1150.0105.149071
GGAACAA1450.0102.347283
Produced by FastQC (version 0.11.5)