FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA3_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA3_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731257
Sequences flagged as poor quality0
Sequence length116
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1345762.3481061833381403No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT283960.495458500639563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164130.2863769675657539No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128160.2236158664669897No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.10263019089878538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT186150.087.729171
AGCAGTG198650.082.0758742
GTATCAA239350.068.36739
TGGTATC239300.068.3586047
CAGTGGT243500.067.1328664
GGTATCA244600.066.922378
AGTGGTA245050.066.8111345
GTGGTAT254250.064.252596
GCAGTGG263000.062.4682963
ATCAACG229550.038.9606110-11
CAACGCA236400.037.8429712-13
ACGCAGA236200.037.64222314-15
AGAGTAC236100.037.55336818-19
GCAGAGT248600.035.8199516-17
TCAACGC232600.035.25808712-13
AGTACTT249900.035.05053720-21
AACGCAG237300.034.43231614-15
TATCAAC241450.034.02297610-11
GTACTTT241200.033.2486722-23
GAGTACT243550.033.0858920-21
Produced by FastQC (version 0.11.5)