FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA2_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA2_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5584290
Sequences flagged as poor quality0
Sequence length116
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1319612.363075699865157No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT275850.49397506218337517No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182040.32598593554417843No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123700.22151428382121988No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57440.10285998757227867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT183100.087.519981
AGCAGTG192150.083.0888062
GTATCAA234600.068.1807569
CAGTGGT237000.067.185364
TGGTATC237550.067.010017
GGTATCA239950.066.477268
AGTGGTA240650.066.3420645
GTGGTAT253200.062.8681956
GCAGTGG256850.062.3305133
ATCAACG227450.038.8607510-11
CAACGCA233500.037.86530312-13
AGAGTAC233850.037.47944618-19
ACGCAGA236050.037.4096614-15
GCAGAGT242500.036.278616-17
AGTACTT249600.034.81706220-21
TCAACGC231500.034.73658412-13
AACGCAG235400.034.1143714-15
TATCAAC238700.033.68911410-11
GTACTTT240400.032.78728522-23
GAGTACT242700.032.66914420-21
Produced by FastQC (version 0.11.5)