FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA2_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA2_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length116
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACAGCAATAGCACTACAGGTAAATGATAAACAAAACAAAACAGA216.666666666666664No Hit
CATTAGAGTCTGGCTCCCATAGAAGGAAGACTAGGCCTCGTGCAGCTATC18.333333333333332No Hit
AGCTTCTCCGTTATCACCCAACTTCATGTGAAGATCCACTGCACTGCCGT18.333333333333332No Hit
ATGCCCTTCTGAAACTTCCGGGCTTGCAGATTTCCTGACAAAGGCCCAGG18.333333333333332No Hit
ATTCCTCATTCCTGACCCATATAAGAAAAACTTTGAGTTGGCTACCTTAA18.333333333333332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18.333333333333332No Hit
ACGCGGCGGTTGGCACCCATATAAGAAATTAAGGTCGTGAGTTCCACGCC18.333333333333332No Hit
CATTAGAGTCTGCCACCGCTGAAGGAAAGCTAGGCGAGTGGAGATTGACG18.333333333333332No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18.333333333333332No Hit
GGCTGAACACGGCTGACCAGGCCGAACCCCACCTATGTCACTTAACTATC18.333333333333332No Hit
CAAAAAACAAAAATGAAGCTTGGTGTACAGGTGGTCATTACAGACCCTGA18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)