FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA1_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA1_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052871
Sequences flagged as poor quality0
Sequence length116
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT718732.3542756965492484No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155190.5083411647593363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99120.3246779834457466No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67920.22247910245798136No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.10757087344994269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99350.087.439341
AGCAGTG106850.081.130822
CAGTGGT128500.067.247374
GTATCAA129450.066.813669
TGGTATC129500.066.702957
AGTGGTA129700.066.7023545
GGTATCA131550.065.538098
GTGGTAT137600.062.936236
GCAGTGG143100.060.4761053
ATCAACG124000.038.17860410-11
ACGCAGA127950.036.95700514-15
CAACGCA128150.036.9422312-13
AGAGTAC128950.036.43588618-19
GCAGAGT134600.035.0902716-17
TCAACGC126750.034.37873512-13
AGTACTT136000.034.2438920-21
AACGCAG128550.033.7690314-15
TATCAAC131200.033.17077610-11
GTACTTT130900.032.55372222-23
CGCAGAG134500.032.2751616-17
Produced by FastQC (version 0.11.5)