FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_cDNA1_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_cDNA1_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length116
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCATCCAACCCATATAAGAAAAACGTAGGAGCATCATAACCAGGGA12.857142857142857No Hit
CATTAGAGTCTGCCACCCATATAAGAAATCAGTGCCCATAGCCCACTTAT12.857142857142857No Hit
ACGGAACAAGAACCCATATAAGAAACCGGGTTTCCACGAGCAACGAAAGG12.857142857142857No Hit
CGGTTCTTTTGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.857142857142857No Hit
ACGCAGTATTTCCGACCCATATAAGAAAAGTGCAAGCAATATCACCTCTG12.857142857142857No Hit
GCATAGCTCTTAAACGGGGACAGATAAGGCTTCTACCCCATATAAGAAAG12.857142857142857No Hit
GCATAGCTCTTAAACGGTGTGAAAGTTAGCAGATACCCCATATAAGAACC12.857142857142857No Hit
CATTAGAGTCTGCCACCCATATAAGAAAGAAAATACTCCTGTGTGCTATG12.857142857142857No Hit
ATTCGTGGTTCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.857142857142857No Hit
ACGGAACAAGAACCCATATAAGAAAGTATTAAGCTATCCTGGCTAAGTTC12.857142857142857No Hit
GCATAGCTCTTAAACAACCATAGTATTTGATGGTCCCCCATATAAGAAAA12.857142857142857No Hit
GATTCCTTTACGAGCCCCATATAAGAAACATCCCATATAAGAAACGGGAT12.857142857142857No Hit
CATTAGAGTCTGCCACCCATATAAGAAAGCGGAGCTGTTTACGTACTCAA12.857142857142857No Hit
ATTCCTCATTCCTGACCCATATAAGAAATCAGTGTCACATCATGGACCAG12.857142857142857No Hit
ACTGCCATATCCCTACCCATATAAGAAAGGTTATGAAACATTCATTGACG12.857142857142857No Hit
CATTAGAGTCTGCCGCCCAGGGGGGGGGGGCGCGGTCGGCGTGGGGGGGG12.857142857142857No Hit
GCGTAGTCGCGTAGTCCCATATAAGAGAGGCAGACGTGAGTGGTCACGGA12.857142857142857No Hit
GCATAGCTCTTAAACAATCTGCTAGTTTTGGGTACCCCCATATAAGAAAA12.857142857142857No Hit
ACGGAACAAGAACCCATATAAGAAAATGCAAGTTGTCACGGCGATCACTA12.857142857142857No Hit
GCATAGCTCTTAAACAGAAGCAGTGCGTCCCGTTACCCCATATAAGAAAC12.857142857142857No Hit
AGGTGCTTTGTGGCATCTCACAAGGAAGTCAGCACCTTCTTGACATGTTC12.857142857142857No Hit
CATTAGAGTCTGCCACCCATATAAGAAACGAAACCTCGAGGAGCAACTGT12.857142857142857No Hit
ACGTAGTCACCTAGTCCCATATAAGAAATTAAGGTTAGTCTAAGCGACGT12.857142857142857No Hit
ACCCGTCCCTAAGAACCCATATAAGAAAAAGACACCCGCACAAGGGAAAC12.857142857142857No Hit
GCATAGCTCTTAAACAACTTTGTTGGGCTCTCACCCCATATAAGAAAGTT12.857142857142857No Hit
ACATATTACCGTCCCATATAATAAAGAGCGAGTCAATCATAACACAGAAA12.857142857142857No Hit
CAACTTGGCCGAATCCCCATATAAGAAACCGTGGTTTAAAGTCGGGAGTG12.857142857142857No Hit
GCATAGCTCTTAAACCATGTACGCAGACATACAACCACCATATAAGAAAT12.857142857142857No Hit
GCATAGTCACCTAGTCCCATATAAGAAAACGTCTGCGGATACGGTACTCT12.857142857142857No Hit
ACTGCCATATCCCTACCCATATAAGAAAATGCGCCCCATCTAAGCGACGT12.857142857142857No Hit
ATTCCTCATTCCTGACCCATATAAGAAACGAGCATCGGATGCACTGACCC12.857142857142857No Hit
ACATGTTACCGTCCCATATAAGAAAAGTCTTAAATAAACGCGGAATCATG12.857142857142857No Hit
ACCCGTCCCGCAGAGCGCTTAGAAGAAGGGAGAACTCCGCGGGTGGGATG12.857142857142857No Hit
GCGTCCGGGCCGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.857142857142857No Hit
GCATTCATTTGTGGCCCCATATAAGAAAAAGCGTATGTGTACTAGTGACT12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)